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revision 1.22, Fri Sep 9 21:10:46 2005 UTC revision 1.45, Thu Oct 20 10:20:27 2005 UTC
# Line 70  Line 70 
70    
71  * B<maxSequenceLength> maximum number of residues per sequence, (default C<8000>)  * B<maxSequenceLength> maximum number of residues per sequence, (default C<8000>)
72    
73    * B<noDBOpen> suppresses the connection to the database if TRUE, else FALSE
74    
75  =back  =back
76    
77  For example, the following constructor call specifies a database named I<Sprout> and a user name of  For example, the following constructor call specifies a database named I<Sprout> and a user name of
# Line 98  Line 100 
100                                                          # database connection port                                                          # database connection port
101                         maxSegmentLength => 4500,        # maximum feature segment length                         maxSegmentLength => 4500,        # maximum feature segment length
102                         maxSequenceLength => 8000,       # maximum contig sequence length                         maxSequenceLength => 8000,       # maximum contig sequence length
103                           noDBOpen     => 0,               # 1 to suppress the database open
104                        }, $options);                        }, $options);
105      # Get the data directory.      # Get the data directory.
106      my $dataDir = $optionTable->{dataDir};      my $dataDir = $optionTable->{dataDir};
# Line 105  Line 108 
108      $optionTable->{userData} =~ m!([^/]*)/(.*)$!;      $optionTable->{userData} =~ m!([^/]*)/(.*)$!;
109      my ($userName, $password) = ($1, $2);      my ($userName, $password) = ($1, $2);
110      # Connect to the database.      # Connect to the database.
111      my $dbh = DBKernel->new($optionTable->{dbType}, $dbName, $userName, $password, $optionTable->{port});      my $dbh;
112        if (! $optionTable->{noDBOpen}) {
113            $dbh = DBKernel->new($optionTable->{dbType}, $dbName, $userName,
114                                    $password, $optionTable->{port});
115        }
116      # Create the ERDB object.      # Create the ERDB object.
117      my $xmlFileName = "$optionTable->{xmlFileName}";      my $xmlFileName = "$optionTable->{xmlFileName}";
118      my $erdb = ERDB->new($dbh, $xmlFileName);      my $erdb = ERDB->new($dbh, $xmlFileName);
# Line 382  Line 389 
389    
390  =head3 LoadUpdate  =head3 LoadUpdate
391    
392  C<< my %stats = $sprout->LoadUpdate($truncateFlag, \@tableList); >>  C<< my $stats = $sprout->LoadUpdate($truncateFlag, \@tableList); >>
393    
394  Load updates to one or more database tables. This method enables the client to make changes to one  Load updates to one or more database tables. This method enables the client to make changes to one
395  or two tables without reloading the whole database. For each table, there must be a corresponding  or two tables without reloading the whole database. For each table, there must be a corresponding
# Line 603  Line 610 
610          if ($prevContig eq $contigID && $dir eq $prevDir) {          if ($prevContig eq $contigID && $dir eq $prevDir) {
611              # Here the new segment is in the same direction on the same contig. Insure the              # Here the new segment is in the same direction on the same contig. Insure the
612              # new segment's beginning is next to the old segment's end.              # new segment's beginning is next to the old segment's end.
613              if (($dir eq "-" && $beg == $prevBeg - $prevLen) ||              if ($dir eq "-" && $beg + $len == $prevBeg) {
614                  ($dir eq "+" && $beg == $prevBeg + $prevLen)) {                  # Here we're merging two backward blocks, so we keep the new begin point
615                  # Here we need to merge two segments. Adjust the beginning and length values                  # and adjust the length.
616                  # to include both segments.                  $len += $prevLen;
617                    # Pop the old segment off. The new one will replace it later.
618                    pop @retVal;
619                } elsif ($dir eq "+" && $beg == $prevBeg + $prevLen) {
620                    # Here we need to merge two forward blocks. Adjust the beginning and
621                    # length values to include both segments.
622                  $beg = $prevBeg;                  $beg = $prevBeg;
623                  $len += $prevLen;                  $len += $prevLen;
624                  # Pop the old segment off. The new one will replace it later.                  # Pop the old segment off. The new one will replace it later.
# Line 615  Line 627 
627          }          }
628          # Remember this specifier for the adjacent-segment test the next time through.          # Remember this specifier for the adjacent-segment test the next time through.
629          ($prevContig, $prevBeg, $prevDir, $prevLen) = ($contigID, $beg, $dir, $len);          ($prevContig, $prevBeg, $prevDir, $prevLen) = ($contigID, $beg, $dir, $len);
630            # Compute the initial base pair.
631            my $start = ($dir eq "+" ? $beg : $beg + $len - 1);
632          # Add the specifier to the list.          # Add the specifier to the list.
633          push @retVal, "${contigID}_$beg$dir$len";          push @retVal, "${contigID}_$start$dir$len";
634      }      }
635      # Return the list in the format indicated by the context.      # Return the list in the format indicated by the context.
636      return (wantarray ? @retVal : join(',', @retVal));      return (wantarray ? @retVal : join(',', @retVal));
# Line 650  Line 664 
664      shift if UNIVERSAL::isa($_[0],__PACKAGE__);      shift if UNIVERSAL::isa($_[0],__PACKAGE__);
665      my ($location) = @_;      my ($location) = @_;
666      # Parse it into segments.      # Parse it into segments.
667      $location =~ /^(.*)_(\d*)([+-_])(\d*)$/;      $location =~ /^(.+)_(\d+)([+\-_])(\d+)$/;
668      my ($contigID, $start, $dir, $len) = ($1, $2, $3, $4);      my ($contigID, $start, $dir, $len) = ($1, $2, $3, $4);
669      # If the direction is an underscore, convert it to a + or -.      # If the direction is an underscore, convert it to a + or -.
670      if ($dir eq "_") {      if ($dir eq "_") {
# Line 758  Line 772 
772          # the start point is the ending. Note that in the latter case we must reverse the DNA string          # the start point is the ending. Note that in the latter case we must reverse the DNA string
773          # before putting it in the return value.          # before putting it in the return value.
774          my ($start, $stop);          my ($start, $stop);
775            Trace("Parse of \"$location\" is $beg$dir$len.") if T(SDNA => 4);
776          if ($dir eq "+") {          if ($dir eq "+") {
777              $start = $beg;              $start = $beg;
778              $stop = $beg + $len - 1;              $stop = $beg + $len - 1;
779          } else {          } else {
780              $start = $beg + $len + 1;              $start = $beg - $len + 1;
781              $stop = $beg;              $stop = $beg;
782          }          }
783            Trace("Looking for sequences containing $start through $stop.") if T(SDNA => 4);
784          my $query = $self->Get(['IsMadeUpOf','Sequence'],          my $query = $self->Get(['IsMadeUpOf','Sequence'],
785              "IsMadeUpOf(from-link) = ? AND IsMadeUpOf(start-position) + IsMadeUpOf(len) > ? AND " .              "IsMadeUpOf(from-link) = ? AND IsMadeUpOf(start-position) + IsMadeUpOf(len) > ? AND " .
786              " IsMadeUpOf(start-position) <= ? ORDER BY IsMadeUpOf(start-position)",              " IsMadeUpOf(start-position) <= ? ORDER BY IsMadeUpOf(start-position)",
# Line 776  Line 792 
792                  $sequence->Values(['IsMadeUpOf(start-position)', 'Sequence(sequence)',                  $sequence->Values(['IsMadeUpOf(start-position)', 'Sequence(sequence)',
793                                     'IsMadeUpOf(len)']);                                     'IsMadeUpOf(len)']);
794              my $stopPosition = $startPosition + $sequenceLength;              my $stopPosition = $startPosition + $sequenceLength;
795                Trace("Sequence is from $startPosition to $stopPosition.") if T(SDNA => 4);
796              # Figure out the start point and length of the relevant section.              # Figure out the start point and length of the relevant section.
797              my $pos1 = ($start < $startPosition ? 0 : $start - $startPosition);              my $pos1 = ($start < $startPosition ? 0 : $start - $startPosition);
798              my $len = ($stopPosition <= $stop ? $stopPosition : $stop) - $startPosition - $pos1;              my $len1 = ($stopPosition < $stop ? $stopPosition : $stop) + 1 - $startPosition - $pos1;
799                Trace("Position is $pos1 for length $len1.") if T(SDNA => 4);
800              # Add the relevant data to the location data.              # Add the relevant data to the location data.
801              $locationDNA .= substr($sequenceData, $pos1, $len);              $locationDNA .= substr($sequenceData, $pos1, $len1);
802          }          }
803          # Add this location's data to the return string. Note that we may need to reverse it.          # Add this location's data to the return string. Note that we may need to reverse it.
804          if ($dir eq '+') {          if ($dir eq '+') {
805              $retVal .= $locationDNA;              $retVal .= $locationDNA;
806          } else {          } else {
807              $locationDNA = join('', reverse split //, $locationDNA);              $retVal .= FIG::reverse_comp($locationDNA);
             $retVal .= $locationDNA;  
808          }          }
809      }      }
810      # Return the result.      # Return the result.
# Line 857  Line 874 
874      # Set it from the sequence data, if any.      # Set it from the sequence data, if any.
875      if ($sequence) {      if ($sequence) {
876          my ($start, $len) = $sequence->Values(['IsMadeUpOf(start-position)', 'IsMadeUpOf(len)']);          my ($start, $len) = $sequence->Values(['IsMadeUpOf(start-position)', 'IsMadeUpOf(len)']);
877          $retVal = $start + $len;          $retVal = $start + $len - 1;
878        }
879        # Return the result.
880        return $retVal;
881    }
882    
883    =head3 ClusterPEGs
884    
885    C<< my $clusteredList = $sprout->ClusterPEGs($sub, \@pegs); >>
886    
887    Cluster the PEGs in a list according to the cluster coding scheme of the specified
888    subsystem. In order for this to work properly, the subsystem object must have
889    been used recently to retrieve the PEGs using the B<get_pegs_from_cell> method.
890    This causes the cluster numbers to be pulled into the subsystem's color hash.
891    If a PEG is not found in the color hash, it will not appear in the output
892    sequence.
893    
894    =over 4
895    
896    =item sub
897    
898    Sprout subsystem object for the relevant subsystem, from the L</get_subsystem>
899    method.
900    
901    =item pegs
902    
903    Reference to the list of PEGs to be clustered.
904    
905    =item RETURN
906    
907    Returns a list of the PEGs, grouped into smaller lists by cluster number.
908    
909    =back
910    
911    =cut
912    #: Return Type $@@;
913    sub ClusterPEGs {
914        # Get the parameters.
915        my ($self, $sub, $pegs) = @_;
916        # Declare the return variable.
917        my $retVal = [];
918        # Loop through the PEGs, creating arrays for each cluster.
919        for my $pegID (@{$pegs}) {
920            my $clusterNumber = $sub->get_cluster_number($pegID);
921            # Only proceed if the PEG is in a cluster.
922            if ($clusterNumber >= 0) {
923                # Push this PEG onto the sub-list for the specified cluster number.
924                push @{$retVal->[$clusterNumber]}, $pegID;
925            }
926      }      }
927      # Return the result.      # Return the result.
928      return $retVal;      return $retVal;
# Line 1007  Line 1072 
1072    
1073  =head3 FeatureAnnotations  =head3 FeatureAnnotations
1074    
1075  C<< my @descriptors = $sprout->FeatureAnnotations($featureID); >>  C<< my @descriptors = $sprout->FeatureAnnotations($featureID, $rawFlag); >>
1076    
1077  Return the annotations of a feature.  Return the annotations of a feature.
1078    
# Line 1017  Line 1082 
1082    
1083  ID of the feature whose annotations are desired.  ID of the feature whose annotations are desired.
1084    
1085    =item rawFlag
1086    
1087    If TRUE, the annotation timestamps will be returned in raw form; otherwise, they
1088    will be returned in human-readable form.
1089    
1090  =item RETURN  =item RETURN
1091    
1092  Returns a list of annotation descriptors. Each descriptor is a hash with the following fields.  Returns a list of annotation descriptors. Each descriptor is a hash with the following fields.
1093    
1094  * B<featureID> ID of the relevant feature.  * B<featureID> ID of the relevant feature.
1095    
1096  * B<timeStamp> time the annotation was made, in user-friendly format.  * B<timeStamp> time the annotation was made.
1097    
1098  * B<user> ID of the user who made the annotation  * B<user> ID of the user who made the annotation
1099    
# Line 1035  Line 1105 
1105  #: Return Type @%;  #: Return Type @%;
1106  sub FeatureAnnotations {  sub FeatureAnnotations {
1107      # Get the parameters.      # Get the parameters.
1108      my ($self, $featureID) = @_;      my ($self, $featureID, $rawFlag) = @_;
1109      # Create a query to get the feature's annotations and the associated users.      # Create a query to get the feature's annotations and the associated users.
1110      my $query = $self->Get(['IsTargetOfAnnotation', 'Annotation', 'MadeAnnotation'],      my $query = $self->Get(['IsTargetOfAnnotation', 'Annotation', 'MadeAnnotation'],
1111                             "IsTargetOfAnnotation(from-link) = ?", [$featureID]);                             "IsTargetOfAnnotation(from-link) = ?", [$featureID]);
# Line 1048  Line 1118 
1118              $annotation->Values(['IsTargetOfAnnotation(from-link)',              $annotation->Values(['IsTargetOfAnnotation(from-link)',
1119                                   'Annotation(time)', 'MadeAnnotation(from-link)',                                   'Annotation(time)', 'MadeAnnotation(from-link)',
1120                                   'Annotation(annotation)']);                                   'Annotation(annotation)']);
1121            # Convert the time, if necessary.
1122            if (! $rawFlag) {
1123                $timeStamp = FriendlyTimestamp($timeStamp);
1124            }
1125          # Assemble them into a hash.          # Assemble them into a hash.
1126          my $annotationHash = { featureID => $featureID,          my $annotationHash = { featureID => $featureID,
1127                                 timeStamp => FriendlyTimestamp($timeStamp),                                 timeStamp => $timeStamp,
1128                                 user => $user, text => $text };                                 user => $user, text => $text };
1129          # Add it to the return list.          # Add it to the return list.
1130          push @retVal, $annotationHash;          push @retVal, $annotationHash;
# Line 1126  Line 1200 
1200  The functional assignment is handled differently depending on the type of feature. If  The functional assignment is handled differently depending on the type of feature. If
1201  the feature is identified by a FIG ID (begins with the string C<fig|>), then a functional  the feature is identified by a FIG ID (begins with the string C<fig|>), then a functional
1202  assignment is a type of annotation. The format of an assignment is described in  assignment is a type of annotation. The format of an assignment is described in
1203  L</ParseLocation>. Its worth noting that we cannot filter on the content of the  L</ParseAssignment>. Its worth noting that we cannot filter on the content of the
1204  annotation itself because it's a text field; however, this is not a big problem because  annotation itself because it's a text field; however, this is not a big problem because
1205  most features only have a small number of annotations.  most features only have a small number of annotations.
1206    
# Line 1217  Line 1291 
1291      return $retVal;      return $retVal;
1292  }  }
1293    
1294    =head3 FunctionsOf
1295    
1296    C<< my @functionList = $sprout->FunctionOf($featureID, $userID); >>
1297    
1298    Return the functional assignments of a particular feature.
1299    
1300    The functional assignment is handled differently depending on the type of feature. If
1301    the feature is identified by a FIG ID (begins with the string C<fig|>), then a functional
1302    assignment is a type of annotation. The format of an assignment is described in
1303    L</ParseAssignment>. Its worth noting that we cannot filter on the content of the
1304    annotation itself because it's a text field; however, this is not a big problem because
1305    most features only have a small number of annotations.
1306    
1307    If the feature is B<not> identified by a FIG ID, then the functional assignment
1308    information is taken from the B<ExternalAliasFunc> table. If the table does
1309    not contain an entry for the feature, an empty list is returned.
1310    
1311    =over 4
1312    
1313    =item featureID
1314    
1315    ID of the feature whose functional assignments are desired.
1316    
1317    =item RETURN
1318    
1319    Returns a list of 2-tuples, each consisting of a user ID and the text of an assignment by
1320    that user.
1321    
1322    =back
1323    
1324    =cut
1325    #: Return Type @@;
1326    sub FunctionsOf {
1327        # Get the parameters.
1328        my ($self, $featureID) = @_;
1329        # Declare the return value.
1330        my @retVal = ();
1331        # Determine the ID type.
1332        if ($featureID =~ m/^fig\|/) {
1333            # Here we have a FIG feature ID. We must build the list of trusted
1334            # users.
1335            my %trusteeTable = ();
1336            # Build a query for all of the feature's annotations, sorted by date.
1337            my $query = $self->Get(['IsTargetOfAnnotation', 'Annotation'],
1338                                   "IsTargetOfAnnotation(from-link) = ? ORDER BY Annotation(time) DESC",
1339                                   [$featureID]);
1340            my $timeSelected = 0;
1341            # Loop until we run out of annotations.
1342            while (my $annotation = $query->Fetch()) {
1343                # Get the annotation text.
1344                my ($text, $time) = $annotation->Values(['Annotation(annotation)','Annotation(time)']);
1345                # Check to see if this is a functional assignment for a trusted user.
1346                my ($user, $function) = _ParseAssignment($text);
1347                if ($user) {
1348                    # Here it is a functional assignment.
1349                    push @retVal, [$user, $function];
1350                }
1351            }
1352        } else {
1353            # Here we have a non-FIG feature ID. In this case the user ID does not
1354            # matter. We simply get the information from the External Alias Function
1355            # table.
1356            push @retVal, $self->GetEntityValues('ExternalAliasFunc', $featureID, ['ExternalAliasFunc(func)']);
1357        }
1358        # Return the assignments found.
1359        return @retVal;
1360    }
1361    
1362  =head3 BBHList  =head3 BBHList
1363    
1364  C<< my $bbhHash = $sprout->BBHList($genomeID, \@featureList); >>  C<< my $bbhHash = $sprout->BBHList($genomeID, \@featureList); >>
# Line 1254  Line 1396 
1396          my $query = $self->Get(['IsBidirectionalBestHitOf'],          my $query = $self->Get(['IsBidirectionalBestHitOf'],
1397                                 "IsBidirectionalBestHitOf(from-link) = ? AND IsBidirectionalBestHitOf(genome) = ?",                                 "IsBidirectionalBestHitOf(from-link) = ? AND IsBidirectionalBestHitOf(genome) = ?",
1398                                 [$featureID, $genomeID]);                                 [$featureID, $genomeID]);
1399          # Look for the best hit.          # Peel off the BBHs found.
1400          my $bbh = $query->Fetch;          my @found = ();
1401          if ($bbh) {          while (my $bbh = $query->Fetch) {
1402              my ($targetFeature) = $bbh->Value('IsBidirectionalBestHitOf(to-link)');              push @found, $bbh->Value('IsBidirectionalBestHitOf(to-link)');
             $retVal{$featureID} = $targetFeature;  
1403          }          }
1404            $retVal{$featureID} = \@found;
1405      }      }
1406      # Return the mapping.      # Return the mapping.
1407      return \%retVal;      return \%retVal;
# Line 1588  Line 1730 
1730                                   [$retVal], ["ParticipatesInCoupling(from-link)", "Coupling(score)"]);                                   [$retVal], ["ParticipatesInCoupling(from-link)", "Coupling(score)"]);
1731      # Check to see if we found anything.      # Check to see if we found anything.
1732      if (!@pegs) {      if (!@pegs) {
1733            Trace("No coupling found.") if T(Coupling => 4);
1734          # No coupling, so undefine the return value.          # No coupling, so undefine the return value.
1735          $retVal = undef;          $retVal = undef;
1736      } else {      } else {
1737          # We have a coupling! Get the score and check for inversion.          # We have a coupling! Get the score and check for inversion.
1738          $score = $pegs[0]->[1];          $score = $pegs[0]->[1];
1739          $inverted = ($pegs[0]->[0] eq $peg1);          my $firstFound = $pegs[0]->[0];
1740            $inverted = ($firstFound ne $peg1);
1741            Trace("Coupling score is $score. First peg is $firstFound, peg 1 is $peg1.") if T(Coupling => 4);
1742      }      }
1743      # Return the result.      # Return the result.
1744      return ($retVal, $inverted, $score);      return ($retVal, $inverted, $score);
# Line 1698  Line 1843 
1843          if ($line =~ m/^>\s*(.+?)(\s|\n)/) {          if ($line =~ m/^>\s*(.+?)(\s|\n)/) {
1844              # Here we have a new header. Store the current sequence if we have one.              # Here we have a new header. Store the current sequence if we have one.
1845              if ($id) {              if ($id) {
1846                  $retVal{$id} = uc $sequence;                  $retVal{$id} = lc $sequence;
1847              }              }
1848              # Clear the sequence accumulator and save the new ID.              # Clear the sequence accumulator and save the new ID.
1849              ($id, $sequence) = ("$prefix$1", "");              ($id, $sequence) = ("$prefix$1", "");
1850          } else {          } else {
1851              # Here we have a data line, so we add it to the sequence accumulator.              # Here we have a data line, so we add it to the sequence accumulator.
1852              # First, we get the actual data out. Note that we normalize to upper              # First, we get the actual data out. Note that we normalize to lower
1853              # case.              # case.
1854              $line =~ /^\s*(.*?)(\s|\n)/;              $line =~ /^\s*(.*?)(\s|\n)/;
1855              $sequence .= $1;              $sequence .= $1;
# Line 1712  Line 1857 
1857      }      }
1858      # Flush out the last sequence (if any).      # Flush out the last sequence (if any).
1859      if ($sequence) {      if ($sequence) {
1860          $retVal{$id} = uc $sequence;          $retVal{$id} = lc $sequence;
1861      }      }
1862      # Close the file.      # Close the file.
1863      close FASTAFILE;      close FASTAFILE;
# Line 2038  Line 2183 
2183      # Get the parameters.      # Get the parameters.
2184      my ($self, $entityName, $entityID) = @_;      my ($self, $entityName, $entityID) = @_;
2185      # Check for the entity instance.      # Check for the entity instance.
2186        Trace("Checking existence of $entityName with ID=$entityID.") if T(4);
2187      my $testInstance = $self->GetEntity($entityName, $entityID);      my $testInstance = $self->GetEntity($entityName, $entityID);
2188      # Return an existence indicator.      # Return an existence indicator.
2189      my $retVal = ($testInstance ? 1 : 0);      my $retVal = ($testInstance ? 1 : 0);
# Line 2531  Line 2677 
2677                                      ['HasSSCell(from-link)', 'IsRoleOf(from-link)']);                                      ['HasSSCell(from-link)', 'IsRoleOf(from-link)']);
2678      # Create the return value.      # Create the return value.
2679      my %retVal = ();      my %retVal = ();
2680        # Build a hash to weed out duplicates. Sometimes the same PEG and role appears
2681        # in two spreadsheet cells.
2682        my %dupHash = ();
2683      # Loop through the results, adding them to the hash.      # Loop through the results, adding them to the hash.
2684      for my $record (@subsystems) {      for my $record (@subsystems) {
2685            # Get this subsystem and role.
2686          my ($subsys, $role) = @{$record};          my ($subsys, $role) = @{$record};
2687          if (exists $retVal{$subsys}) {          # Insure it's the first time for both.
2688            my $dupKey = "$subsys\n$role";
2689            if (! exists $dupHash{"$subsys\n$role"}) {
2690                $dupHash{$dupKey} = 1;
2691              push @{$retVal{$subsys}}, $role;              push @{$retVal{$subsys}}, $role;
         } else {  
             $retVal{$subsys} = [$role];  
2692          }          }
2693      }      }
2694      # Return the hash.      # Return the hash.
# Line 3106  Line 3257 
3257      # Declare the return value.      # Declare the return value.
3258      my @retVal = ();      my @retVal = ();
3259      # Check to see if this is a functional assignment.      # Check to see if this is a functional assignment.
3260      my ($user, $type, $function) = split(/\n/, $text);      my ($type, $function) = split(/\n/, $text);
3261      if ($type =~ m/^set ([^ ]+) function to$/i) {      if ($type =~ m/^set ([^ ]+) function to$/i) {
3262          # Here it is, so we return the user name (which is in $1), the functional role text,          # Here it is, so we return the user name (which is in $1), the functional role text,
3263          # and the assigning user.          # and the assigning user.
3264          @retVal = ($1, $function, $user);          @retVal = ($1, $function);
3265      }      }
3266      # Return the result list.      # Return the result list.
3267      return @retVal;      return @retVal;
# Line 3138  Line 3289 
3289    
3290  sub FriendlyTimestamp {  sub FriendlyTimestamp {
3291      my ($timeValue) = @_;      my ($timeValue) = @_;
3292      my $retVal = strftime("%a %b %e %H:%M:%S %Y", localtime($timeValue));      my $retVal = localtime($timeValue);
3293      return $retVal;      return $retVal;
3294  }  }
3295    

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