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Revision 1.3 - (download) (as text) (annotate)
Wed Aug 10 00:19:00 2011 UTC (8 years, 7 months ago) by overbeek
Branch: MAIN
CVS Tags: rast_rel_2011_0928, mgrast_dev_10262011
Changes since 1.2: +20 -8 lines
make svr_md5_of_prot more bulletproof

use strict;
use Data::Dumper;
use Carp;

# This is a SAS Component

use Digest::MD5;

=head1 svr_md5_of_prot

Get md5s of protein-encoding genes



    svr_all_features 3702.1 peg | svr_md5_of_prot

would produce a 2-column table.  The first column would contain
PEG IDs for genes occurring in genome 3702.1, and the second
would contain the md5 values of the encoded genes.


The standard input should be a tab-separated table (i.e., each line 
is a tab-separated set of fields).  Normally, the last field in each
line would contain the PEG for which md5s are being requested.
If some other column contains the PEGs, use

    -c N

where N is the column (from 1) that contains the PEG in each case.

This is a pipe command. The input is taken from the standard input, and the
output is to the standard output.

=head2 Command-Line Options

=over 4

=item -c Column

This is used only if the column containing PEGs is not the last.


=head2 Output Format

The standard output is a tab-delimited file. It consists of the input
file with an extra column added (the md5 associated with the PEG).


use SeedEnv;
$ENV{'SAS_DEBUG'} = 1;
my $sapObject = SAPserver->new();
use Getopt::Long;

my $usage = "usage: svr_md5_of [-c column]";

my $column;
my $i = "-";
my $rc  = GetOptions('c=i' => \$column, 'i=s' => \$i);
if (! $rc) { print STDERR $usage; exit }
open my $ih, "<$i";
while (my @tuples = ScriptThing::GetBatch($ih, undef, $column)) {
    my @ids = map { $_->[0] } @tuples;
    print STDERR &Dumper(\@ids); 
    my $md5H = $sapObject->fids_to_proteins(-ids => \@ids);
    for my $tuple (@tuples) {
        my ($id, $line) = @$tuple;
        my $md5 = $md5H->{$id};
        if (! defined $md5) {
	    if ($id)
		my $seqsH = $sapObject->ids_to_sequences(-ids => [$id], -protein => 1);
		if ($seqsH->{$id})
		    my $md5 = Digest::MD5::md5_hex( uc $seqsH->{$id} );
		    if (! $md5)
			print STDERR "$id not found.\n";
			print "$line\t$md5\n";
		    print STDERR "$id not found.\n";
        } else {
            print "$line\t$md5\n";


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